Journal: Cancer Cell International

Article Title: Expression pattern of the apoptosis-stimulating protein of p53 family in p53 + human breast cancer cell lines

doi: 10.1186/1475-2867-13-116

Figure Lengend Snippet: Expression of p53 in both BC cell lines. (A) MDA-MB-231. (B) ZR-75-30. (C) Bcap-37. (D) MCF-7. (E) HBL-100 (×200 magnification).

Article Snippet: After blocking for 1 h at room temperature with PBS buffer containing 6% non-fat milk powder, membranes were incubated with mouse anti-p53 primary antibody (2 μg/mL, DO-7, DAKO) at 4°C overnight and were subsequently incubated with the secondary HRP-IgG antibody at room temperature for 1 h.

Techniques: Expressing

Journal: Cancer Cell International

Article Title: Expression pattern of the apoptosis-stimulating protein of p53 family in p53 + human breast cancer cell lines

doi: 10.1186/1475-2867-13-116

Figure Lengend Snippet: Western-blot result of p53 protein expression in 5 BC cell lines.

Article Snippet: After blocking for 1 h at room temperature with PBS buffer containing 6% non-fat milk powder, membranes were incubated with mouse anti-p53 primary antibody (2 μg/mL, DO-7, DAKO) at 4°C overnight and were subsequently incubated with the secondary HRP-IgG antibody at room temperature for 1 h.

Techniques: Western Blot, Expressing

Journal: Cancer Cell International

Article Title: Expression pattern of the apoptosis-stimulating protein of p53 family in p53 + human breast cancer cell lines

doi: 10.1186/1475-2867-13-116

Figure Lengend Snippet: Apoptosis index of BC cell lines after transfected p53 RNAi (%, x ¯ ±s)

Article Snippet: After blocking for 1 h at room temperature with PBS buffer containing 6% non-fat milk powder, membranes were incubated with mouse anti-p53 primary antibody (2 μg/mL, DO-7, DAKO) at 4°C overnight and were subsequently incubated with the secondary HRP-IgG antibody at room temperature for 1 h.

Techniques: Transfection